CAMARA DE NEUBAUER FUNDAMENTO PDF

INTRODUCCIN La cmara de Neubauer es un instrumento utilizado en medicina y biologia para realizar el recuento de clulas en un medio liquido, que puede ser un cultivo celular, sangre, orina, liquido sinovial, liquido cefalorraquideo, etc, por lo cual el alumno deber desarrollar la habilidad de enfocar de forma correcta la camara de Neubauer. Esta cmara de contaje est adaptada al microscopio de campo claro o al de contraste de fases. Se trata de un portaobjetos que tiene dos zonas ligeramente deprimidas y que en el fondo de las cuales se ha marcado con la ayuda de un diamante una cuadrcula de dimensiones conocidas. Se cubre la cmara con un cubrecmaras que se adhiere por simple tensin superficial. Luego se introduce el lquido a contar, al que generalmente se ha sometido a una dilucin previa con un diluyente, por capilaridad entre la cmara y el cubrecmara; puesto que tiene dos zonas esto permite hacer dos recuentos simultaneamente.

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